Campylobacter fetus Medium

[所属分类:培养基配方] [发布时间:2020-3-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter fetus Medium

培养基配方
Composition per liter:
Proteose peptone...............................................10.0g
NaCl............................................................5.0g
Beef extract....................................................3.0g
Bovine blood..................................................50.0mL
Antibiotic solution...........................................10.0mL
pH 7.2–7.4 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Novobiocin ...................................................2.0mg
Bacitracin....................................................2000U
Preparation of Antibiotic Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except bovine blood
and antibiotic solution, to distilled/deionized water and bring volume
to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile bovine blood and antibiotic solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the isolation and cultivation of Campylobacter fetus.