Campylobacter fetus Medium

[所属分类:培养基配方] [发布时间:2020-3-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter fetus Medium

培养基配方
Composition per 1160.0mL:
Fluid thioglycolate agar.....................................1.0L
Sheep blood, defibrinated.................................150.0mL
Antibiotic solution .......................................10.0mL
pH 7.1 ± 0.2 at 25°C
Fluid Thioglycolate Agar:
Composition per liter:
Agar .......................................................15.0g
Pancreatic digest of casein.................................15.0g
Glucose .....................................................5.5g
Yeast extract................................................5.0g
NaCl.........................................................2.5g
Agar .......................................................0.75g
L- Cystine ..................................................0.5g
Sodium thioglycolate.........................................0.5g
Resazurin ..................................................1.0mg
Preparation of Fluid Thioglycolate Agar: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide................................................0.05g
Novobiocin...................................................5.0mg
Bacitracin.................................................25,000U
Polymyxin B sulfate........................................10,000U
Preparation of Antibiotic Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium:  To 1.0L of cooled, sterile, fluid thiogly-
colate agar, aseptically add 150.0mL of sterile sheep blood and 10.0mL
of sterile antibiotic solution.
Use: For the isolation and cultivation of Campylobacter fetus from
human specimens.