Campylobacter fetus Selective Medium

[所属分类:培养基配方] [发布时间:2020-3-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter fetus Selective Medium

培养基配方
Composition per liter:
Fluid thioglycolate agar.................................1.0L
Sheep blood, defibrinated.............................150.0mL
Antibiotic solution ...................................10.0mL
Fluid Thioglycolate Agar:
Composition per liter:
Agar ...................................................15.0g
Pancreatic digest of casein.............................15.0g
Glucose .................................................5.5g
Yeast extract............................................5.0g
NaCl.....................................................2.5g
Agar ...................................................0.75g
L- Cystine ..............................................0.5g
Sodium thioglycolate.....................................0.5g
Resazurin ..............................................1.0mg
Preparation of Fluid Thioglycolate Agar: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide.................................................0.05g
Cephalothin...................................................0.02g
Novobiocin ...................................................5.0mg
Bacitracin..................................................25,000U
Colistin....................................................10,000U
Preparation of Antibiotic Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: To 1.0L of cooled, sterile, fluid thiogly-
colate agar, aseptically add 150.0mL of sterile sheep blood and 10.0mL
of sterile antibiotic solution.
Use: For the isolation and cultivation of Campylobacter fetus.