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Campylobacter Isolation Agar A

[所属分类:培养基配方] [发布时间:2020-3-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Isolation Agar A

培养基配方
Composition per liter:
Agar ..........................................................12.0g
Beef extract...................................................10.0g
Peptone........................................................10.0g
NaCl............................................................5.0g
Charcoal........................................................4.0g
Casein hydrolysate..............................................3.0g
Yeast extract...................................................2.0g
Sodium deoxycholate.............................................1.0g
FeSO4 .........................................................0.25g
Sodium pyruvate................................................0.25g
Antibiotic solution ..........................................10.0mL
pH 7.4 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide...................................................0.1g
Sodium cefoperazone............................................0.03g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile anti-
biotic solution. Mix thoroughly. Pour into sterile Petri dishes. Swirl
flask while pouring to distribute charcoal.
Use: For the isolation and cultivation of Campylobacter species.

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