Campylobacter Isolation Agar B (Campy Cefex Agar)

[所属分类:培养基配方] [发布时间:2020-3-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Isolation Agar B
(Campy Cefex Agar)

培养基配方
Composition per liter:
Agar..........................................................15.0g
Pancreatic digest of casein...................................10.0g
Peptic digest of animal tissue ...............................10.0g
NaCl...........................................................5.0g
Yeast extract..................................................2.0g
Glucose........................................................1.0g
FeSO4 .........................................................0.5g
Sodium pyruvate................................................0.5g
NaHSO3 .......................................................0.35g
Horse blood, laked...........................................50.0mL
Antibiotic solution..........................................10.0mL
pH 7.0 ± 0.2 at 25°C
Horse Blood, Laked:
Composition per 50.0mL:
Horse blood, fresh...........................................50.0mL
Preparation of Horse Blood, Laked: Add blood to a sterile poly-
propylene bottle. Freeze overnight at −20°C. Thaw at 8°C. Refreeze at
−20°C. Thaw again at 8°C.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide..................................................0.1g
Sodium cefoperazone..........................................0.033g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except horse blood and
antibiotic solution, to distilled/deionized water and bring volume to
940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile horse blood and antibiotic solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Campylobacter species.