Campylobacter Medium

[所属分类:培养基配方] [发布时间:2020-3-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Medium

培养基配方
Composition per liter:
Sodium aspartate...............................................10.0g
MgSO4 ·7H2O....................................................1.0g
K2HPO4 ........................................................0.75g
Yeast extract...................................................0.2g
CaCl2 ·2H2O .................................................28.0mg
Resazurin......................................................1.0mg
Phosphate-cysteine solution..................................100.0mL
Trace elements solution SL-10..................................1.0mL
pH 7.0 ± 0.2 at 25°C
Phosphate-Cysteine Solution:
Composition per 100.0mL:
NaH2PO4 .......................................................0.25g
L -Cysteine·HCl ..............................................0.25g
Preparation of Phosphate-Cysteine Solution : Add components
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O....................................................1.5g
CoCl2 ·6H2O ................................................190.0mg
MnCl2 ·4H2O.................................................100.0mg
ZnCl2 ........................................................70.0mg
Na2MoO4 ·2H2O ...............................................36.0mg
NiCl2 ·6H2O..................................................24.0mg
H3BO3 .........................................................6.0mg
CuCl2 ·2H2O ..................................................2.0mg
HCl (25% solution)............................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly.
Preparation of Medium: Add components, except phosphate-
cysteine solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 100.0mL of sterile phosphate-cysteine solu-
tion. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Campylobacter species,
Actinobacillus ureae, Erysipelothrix rhusiopathiae, Helicobacter
pylori, Moraxella bovis, Moraxella nonliquefaciens, Moraxella
osloensis, Pasteurella haemolytica, and Pasteurella multocida.