Campylobacter rectus Medium

[所属分类:培养基配方] [发布时间:2020-4-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter rectus Medium

培养基配方
Composition per liter:
Yeast extract................................................11.0g
Pancreatic digest of casein...................................9.0g
Beef extract..................................................3.0g
NaCl..........................................................2.0g
Na2HPO4 ......................................................0.4g
Na2CO3 ......................................................0.25g
Resazurin ...................................................1.0mg
Formate-fumarate solution..................................100.0mL
Hemin solution..............................................10.0mL
pH 7.5 ± 0.2 at 25°C
Formate-Fumarate Solution:
Composition per 100.0mL:
Sodium fumarate................................................3.0g
Sodium formate ................................................2.0g
Preparation of Formate-Fumarate Solution : Add components
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Filter sterilize. Sparge with 100% N2 for 3–4 min.
Hemin Solution:
Composition per 10.0mL:
Hemin .........................................................5.0mg
NaOH (1N solution).............................................0.1mL
Preparation of Hemin Solution : Add hemin to NaOH solution to
dissolve. Add distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Sparge with 100% N2 for 3–4 min. Autoclave under
100% N2  for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except formate-fumar-
ate solution and hemin solution, to distilled/deionized water and bring
volume to 890.0mL. Sparge with 100% N2 for 10 min. Autoclave under
100% N2  for 15 min at 15 psi pressure–121°C. Aseptically and anaer-
obically add 100.0mL of sterile formate-fumarate solution and 10.0mL
of sterile hemin solution under 100% N2 . Mix thoroughly. Aseptically
and anaerobically distribute into sterile anaerobic tubes.
Use:  For the cultivation and maintenance of Campylobacter curvus
and Campylobacter rectus.