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Campylobacter Selective Medium, Blaser-Wang (Blaser–Wang Campylobacter Medium) (Blaser’s Agar) (Campy BAP Medium)

[所属分类:培养基配方] [发布时间:2020-4-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Selective Medium, Blaser-Wang
(Blaser–Wang Campylobacter Medium)
(Blaser’s Agar)
(Campy BAP Medium)

培养基配方
Composition per liter:
Brucella agar base.....................................890.0mL
Sheep blood............................................100.0mL
Antibiotic supplement...................................10.0mL
Brucella Agar Base:
Composition per 890.0mL:
Agar ....................................................15.0g
Glucose .................................................10.0g
Pancreatic digest of casein..............................10.0g
NaCl......................................................5.0g
Peptic digest of animal tissue............................5.0g
pH 7.5 ± 0.2 at 25°C
Preparation of Brucella Agar Base: Add components to distilled/
deionized water and bring volume to 890.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Antibiotic Supplement:
Composition per 10.0mL:
Cephalothin...............................................15.0mg
Vancomycin ...............................................10.0mg
Trimethoprim...............................................5.0mg
Amphotericin B.............................................2.0mg
Polymyxin B................................................2500U
Preparation of Antibiotic Supplement: Add components to
10.0mL of distilled/deionized water. Filter sterilize.
Preparation of Medium: Prepare 890.0mL of Brucella agar base.
Sterilize as directed. Cool to 50°–55°C and add 100.0mL of sheep
blood or 50.0–70.0mL of laked horse blood. Laked blood is prepared
by freezing whole blood overnight and thawing to room temperature.
Aseptically add 10.0mL of sterile antibiotic supplement. Mix thor-
oughly. Pour into sterile Petri dishes.
Use:  For the selective isolation of Campylobacter species.

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