Campylobacter Selective Medium, Blaser-Wang (Blaser–Wang Campylobacter Medium) (Blaser’s Agar) (Campy BAP Medium)

[所属分类:培养基配方] [发布时间:2020-4-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Selective Medium, Blaser-Wang
(Blaser–Wang Campylobacter Medium)

培养基配方
Composition per liter:
Columbia agar base.......................................890.0mL
Sheep blood..............................................100.0mL
Antibiotic supplement.....................................10.0mL
pH 7.3 ± 0.2 at 25°C
Antibiotic Supplement:
Composition per 10.0mL:
Cephalothin...............................................15.0mg
Vancomycin ...............................................10.0mg
Trimethoprim...............................................5.0mg
Amphotericin B.............................................2.0mg
Polymyxin B...............................................2,500U
Preparation of Antibiotic Supplement: Add components to
10.0mL of distilled/deionized water. Filter sterilize.
Columbia Agar Base:
Composition per liter:
Special peptone..............................................25.0g
Agar ........................................................10.0g
NaCl..........................................................5.0g
Starch........................................................1.0g
Preparation of Columbia Agar Base: Add components to dis-
tilled/deionized water and bring volume to 890.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C.
Preparation of Medium: To 890.0mL of cooled, sterile Columbia
agar base, aseptically add 100.0mL of sheep blood or 50.0–70.0mL of
laked horse blood. Laked blood is prepared by freezing whole blood
overnight and thawing to room temperature. Aseptically add 10.0mL
of sterile antibiotic supplement. Mix thoroughly. Pour into sterile Petri
dishes.
Use:  For the selective isolation of Campylobacter species.