Campylobacter Selective Medium, Butzler’s (Butzler’s Campylobacter Medium)

[所属分类:培养基配方] [发布时间:2020-4-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Selective Medium, Butzler’s
(Butzler’s Campylobacter Medium)

培养基配方

Composition per liter:
Brucella agar base....................................940.0mL
Sheep or horse blood, defibrinated.....................50.0mL
Antibiotic supplement..................................10.0mL
pH 7.5 ± 0.2 at 25°C
Brucella Agar Base:
Composition per liter:
Agar....................................................15.0g
Glucose.................................................10.0g
Pancreatic digest of casein.............................10.0g
NaCl.....................................................5.0g
Peptic digest of animal tissue ..........................5.0g
Preparation of Brucella Agar Base: Add components to distilled/
deionized water and bring volume to 940.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Antibiotic Supplement:
Composition per 10.0mL:
Cycloheximide..............................................50.0mg
Cephazolin.................................................15.0mg
Novobiocin .................................................5.0mg
Bacitracin................................................25,000U
Colistin sulfate..........................................10,000U
Preparation of Antibiotic Supplement: Add components to
10.0mL of distilled/deionized water. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: To 940.0mL of cooled, sterile Brucella
agar base, aseptically add 50.0mL of defibrinated sheep or horse blood
and 10.0mL of sterile antibiotic supplement. Mix thoroughly. For en-
hanced growth, medium may also be supplemented with 0.25g of
Fe2SO4 ·H2O, 0.25g of sodium metabisulfite, and 0.25g of sodium
pyruvate. Pour into sterile Petri dishes.
Use:  For the selective isolation of Campylobacter species.