Campylobacter Selective Medium, Butzler’s (Butzler’s Campylobacter Medium)

[所属分类:培养基配方] [发布时间:2020-4-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Selective Medium, Butzler’s
(Butzler’s Campylobacter Medium)

培养基配方
Composition per liter:
Columbia agar base....................................940.0mL
Blood, horse or sheep .................................50.0mL
Antibiotic supplement..................................10.0mL
pH 7.3 ± 0.2 at 25°C
Columbia Agar Base:
Composition per liter:
Peptone..................................................25.0g
Agar ....................................................10.0g
NaCl......................................................5.0g
Starch ...................................................1.0g
Preparation of Columbia Agar Base : Add components to dis-
tilled/deionized water and bring volume to 940.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C.
Antibiotic Supplement:
Composition per 10.0mL:
Cycloheximide..............................................50.0mg
Cephazolin.................................................15.0mg
Novobiocin..................................................5.0mg
Bacitracin................................................25,000U
Colistin sulfate..........................................10,000U
Preparation of Antibiotic Supplement: Add components to
10.0mL of distilled/deionized water. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: To 940.0mL of cooled, sterile Columbia
agar base, aseptically add 50.0mL of defibrinated sheep or horse blood and
10.0mL of sterile antibiotic supplement. Mix thoroughly. The medium
may also be supplemented with 0.25g of Fe2SO4 ·H2O, 0.25g of sodium
metabisulfite, and 0.25g of sodium pyruvate. Pour into sterile Petri dishes.
Use:  For the selective isolation of Campylobacter species.