Campylobacter Selective Medium, Karmali’s (Karmali’s Campylobacter Medium)

[所属分类:培养基配方] [发布时间:2020-4-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Selective Medium, Karmali’s
(Karmali’s Campylobacter Medium)

培养基配方

Composition per liter:
Activated charcoal .........................................4.0g
Columbia agar base.......................................990.0mL
Antibiotic supplement.....................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Columbia Agar Base:
Composition per 990.0mL:
Peptone.....................................................25.0g
Agar .......................................................10.0g
NaCl.........................................................5.0g
Starch ......................................................1.0g
Preparation of Columbia Agar Base:  Add components to dis-
tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C.
Antibiotic Supplement:
Composition per 10.0mL:
Sodium pyruvate.............................................0.05g
Cycloheximide...............................................0.05g
Cefoperazone...............................................0.016g
Hemin......................................................0.016g
Vancomycin .................................................0.01g
Preparation of Antibiotic Supplement: Add components to
10.0mL of distilled/deionized water. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Prepare 990.0mL of Columbia agar base.
Sterilize as directed. Cool to 50°–55°C. Add defibrinated sheep or horse
blood to a final concentration of 5–7%. Add 10.0mL of sterile antibiotic
supplement. Mix thoroughly. For enhanced growth, medium may also be
supplemented with 0.25g of Fe2SO4 ·H2O, 0.25g of sodium metabisulfite,
and 0.25g of sodium pyruvate. Pour into sterile Petri dishes. Swirl while
pouring to keep charcoal in suspension.
Use: For the selective isolation of Campylobacter species.