Campylobacter Selective Medium, Preston’s (Preston’s Campylobacter Medium)

[所属分类:培养基配方] [发布时间:2020-4-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Selective Medium, Preston’s
(Preston’s Campylobacter Medium)

培养基配方
Composition per liter:
Campylobacter agar base....................................940.0mL
Horse blood, lysed..........................................50.0mL
Antibiotic supplement.......................................10.0mL
pH 7.5 ± 0.2 at 25°C
Campylobacter Agar Base:
Composition per liter:
Agar..........................................................12.0g
Beef extract..................................................10.0g
Peptone ......................................................10.0g
NaCl...........................................................5.0g
Preparation of Campylobacter Agar Base: Add components to
distilled/deionized water and bring volume to 940.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C.
Antibiotic Supplement:
Composition per 10.0mL:
Cycloheximide..................................................0.1g
Rifampicin....................................................0.01g
Trimethoprim lactate..........................................0.01g
Polmyxin B....................................................5000U
Preparation of Antibiotic Supplement: Add components to
10.0mL of 50:50 acetone:distilled/deionized water. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: To 940.0mL of cooled, sterile Campy-
lobacter agar base, aseptically add 50.0mL of lysed horse blood and
10.0mL of sterile antibiotic supplement. Mix thoroughly. Pour into
sterile Petri dishes.
Use:  For the selective isolation of Campylobacter species.