Campylobacter sputorum subspecies bubulus Medium

[所属分类:培养基配方] [发布时间:2020-4-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter sputorum subspecies bubulus Medium

培养基配方
Composition per liter:
Agar......................................................1.5g
Brilliant Green..........................................0.01g
Ethyl Violet............................................1.25mg
Brucella broth base.......................................1.0L
Antibiotic solution.....................................10.0mL
pH 7.0 ± 0.2 at 25°C
Brucella Broth Base:
Composition per liter:
Pancreatic digest of casein..............................10.0g
Peptic digest of animal tissue ..........................10.0g
NaCl......................................................5.0g
Yeast extract.............................................2.0g
Glucose ..................................................1.0g
NaHSO3 ...................................................0.1g
Preparation of Brucella Broth Base: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide............................................0.1g
Bacitracin............................................20,000U
Preparation of Antibiotic Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: To 1.0L of Brucella broth base, add agar,
Brilliant Green, and Ethyl Violet. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add ster-
ile antibiotic solution. Mix thoroughly. Aseptically distribute into ster-
ile tubes or flasks.
Use: For the cultivation and isolation of Campylobacter sputorum
subspecies bubulus.