Campylobacter sputorum subspecies mucosalis Medium

[所属分类:培养基配方] [发布时间:2020-4-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter sputorum subspecies mucosalis Medium

培养基配方
Composition per liter:
Yeast extract...............................................2.8g
KNO3 .......................................................1.0g
Fluid thioglycolate broth without glucose...................1.0L
Fluid Thioglycolate Broth without Glucose:
Composition per liter:
Pancreatic digest of casein.................................15.0g
Yeast extract................................................5.0g
NaCl.........................................................2.5g
Agar .......................................................0.75g
L- Cystine ..................................................0.5g
Sodium thioglycolate.........................................0.5g
Resazurin ..................................................1.0mg
Preparation of Fluid Thioglycolate Broth without Glucose:
Add components to distilled/deionized water and bring volume to
1.0L. Mix thoroughly.
Preparation of Medium: Combine components. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation and isolation of Campylobacter sputorum
subspecies mucosalis.