Campylobacter Thioglycolate Medium with 5 Antimicrobics

[所属分类:培养基配方] [发布时间:2020-4-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Thioglycolate Medium
with 5 Antimicrobics

培养基配方
Composition per liter:
Pancreatic digest of casein.................................17.0g
Glucose .....................................................6.0g
Papaic digest of soybean meal................................3.0g
NaCl.........................................................2.5g
Agar ........................................................1.6g
Sodium thioglycolate.........................................0.5g
Na2SO3 ......................................................0.1g
Antibiotic supplement solution.............................10.0mL
pH 7.0 ± 0.2 at 25°C
Antibiotic Supplement Solution:
Composition per 10.0mL:
Cephalothin.................................................0.015g
Vancomycin...................................................0.01g
Trimethoprim.................................................5.0mg
Amphotericin B ..............................................2.0mg
Polymyxin B..................................................2500U
Preparation of Antibiotic Supplement Solution: Add compo-
nents to 10.0mL of distilled/deionized water. Mix thoroughly. Filter
sterilize.
Preparation of Medium:  Add components, except cephalothin,
vancomycin, trimethoprim, amphotericin, and polymyxin B, to dis-
tilled deionized water and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Add 10.0mL of sterile antibiotic sup-
plement. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the maintenence—as a holding medium or transport
medium—of fecal specimens or swabs suspected of containing
Campylobacter jejuni or other Campylobacter species when immedi-
ate inoculation of Campylobacter growth medium is unavailable.