Candida BCG Agar Base (Candida Bromcresol Green Agar Base)

[所属分类:培养基配方] [发布时间:2020-4-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Candida BCG Agar Base
(Candida Bromcresol Green Agar Base)

培养基配方
Composition per liter:
Glucose.....................................................40.0g
Agar........................................................15.0g
Peptone ....................................................10.0g
Yeast extract................................................1.0g
Bromcresol Green............................................0.02g
Neomycin solution..........................................10.0mL
pH 6.1 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Neomycin Solution:
Composition per 10.0mL:
Neomycin...................................................0.5g
Preparation of Neomycin Solution: Add neomycin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium:  Add components, except neomycin solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly and heat gently until boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of sterile
neomycin solution. Mix thoroughly. Pour into sterile Petri dishes or
leave in tubes.
Use: For the selective isolation and identification of Candida species.
It is a highly differential medium that is used for demonstrating mor-
phological and biochemical reactions characterizing different Candida
species. Candida albicans appears as blunt conical colonies with
smooth edges and yellow to blue-green color. Candida stellatoidea
appears as convex colonies with smooth edges and yellow to green
color. Candida tropicalis appears as convex colonies with wavy edges
and yellow-green to green color with a dark blue-green base. Candida
pseudotropicalis appears as convex, shiny colonies with smooth edges
and green color with a light green edge. Candida krusei appears as low
conical colonies with spreading edges and blue-green color. Candida
stellatoidea appears as convex colonies with smooth edges and yellow
to green color.