Carbohydrate Fermentation Broth

[所属分类:培养基配方] [发布时间:2020-4-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Carbohydrate Fermentation Broth

培养基配方

Composition per liter:
Peptone ...................................................10.0g
NaCl........................................................5.0g
Meat extract................................................3.0g
Carbohydrate solution.....................................50.0mL
Andrade’s indicator......................................10.0mL
pH 7.1 ± 0.2 at 25°C
Andrade’s Indicator:
Composition per 100.0mL:
Acid Fuchsin................................................0.1g
NaOH (1N solution).........................................16.0mL
Preparation of Andrade’s Indicator:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate.................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Adonitol, ara-
binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lac-
tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,
trehalose, xylose, or other carbohydrates may be used. Mix thoroughly.
Filter sterilize.
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of the powdered dye and contact with the skin.
Preparation of Medium:  Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute in 10.0mL vol-
umes into test tubes containing inverted Durham tubes. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C. Add 0.5mL of sterile
carbohydrate solution to each tube.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms, particularly members of the Enterobacteriaceae. A
Durham tube is used to collect gas produced during the fermentation
reaction. Acid production is indicated by a pink reaction.