​Carbon Assimilation Medium

[所属分类:培养基配方] [发布时间:2020-4-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Carbon Assimilation Medium

培养基配方
Composition per liter:
Agar solution...........................................500.0mL
Mineral base medium.....................................500.0mL
pH 6.5 ± 0.1 at 25°C
Agar Solution:
Composition per liter:
Agar ....................................................32.0g
Preparation of Agar Solution: Add agar to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.
Mineral Base Medium:
Composition per 500.0mL:
Carbohydrate............................................10.0g
NaCl.....................................................5.0g
NH4HPO4 .................................................1.0g
K2HPO4 ..................................................1.0g
MgSO4 ·7H2O, anhydrous..................................0.1g
Preparation of Mineral Base Medium: Add components to dis-
tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Gently heat until dissolved. Filter sterilize. Warm to 45°–50°C.
Preparation of Medium: Combine 500.0mL of cooled, sterile agar
solution and 500.0mL of sterile mineral base medium. Mix thoroughly.
Aseptically distribute into sterile tubes. Allow tubes to cool in a slanted
position.
Use: For the cultivation and differentiation of microorganisms based
on their ability to utilize a particular carbon source.