Carbon Assimilation Medium, Auxanographic Method for Yeast Identification

[所属分类:培养基配方] [发布时间:2020-4-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Carbon Assimilation Medium,
Auxanographic Method for Yeast Identification

培养基配方
Composition per liter:
Noble agar...................................................20.0g
(NH4)2SO4 ....................................................0.5g
KH2PO4 .......................................................0.1g
MgSO4 ·7H2O.................................................0.05g
NaCl.........................................................0.01g
CaCl2 ·2H2O ................................................0.01g
DL- Methionine...............................................2.0mg
DL- Tryptophan...............................................2.0mg
L- Histidine·HCl............................................1.0mg
Inositol.....................................................0.2mg
KI..........................................................0.01mg
H3BO3 ......................................................0.05mg
ZnSO4 ·7H2O................................................0.04mg
MnSO4 ·4H2O................................................0.04mg
Thiamine·HCl...............................................0.04mg
Pyroxidine·HCl.............................................0.04mg
Niacin......................................................0.04mg
Calcium pantothenate .......................................0.04mg
p-Aminobenzoic acid.........................................0.02mg
Riboflavin..................................................0.02mg
FeCl3 ......................................................0.02mg
Na2MoO4 ·4H2O..............................................0.02mg
CuSO4 ·5H2O................................................4.0μg
Folic acid .................................................0.2μg
Biotin......................................................0.2μg
pH 4.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into screw-capped tubes in 20.0mL volumes. Au-
toclave for 15 min at 15 psi pressure–121°C.
Use: For carbohydrate assimilation tests by the auxanographic method
for the identification of yeasts.