Carbon Monoxide Oxidizers Agar, Modified

[所属分类:培养基配方] [发布时间:2020-4-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Carbon Monoxide Oxidizers Agar, Modified

培养基配方
Composition per 1001.0mL:
Agar........................................................12.0g
Na2HPO4 ·12H2O..............................................4.5g
Sodium acetate...............................................3.0g
NH4Cl .......................................................1.5g
KH2PO4 .....................................................0.75g
MgSO4 ·7H2O.................................................0.2g
CaCl2 ·2H2O ..............................................30.0mg
Ferric ammonium citrate....................................18.0mg
Trace elements solution.....................................1.0mL
NaHCO3  solution...........................................10.0mL
Thiamine·HCl solution ....................................10.0mL
Trace Elements Solution:
Composition per liter:
Na2MoO4 ·2H2O..............................................0.9g
H3BO3 ......................................................0.3g
CoCl2 ·6H20................................................0.2g
ZnSO4 ·7H2O................................................0.1g
MnCl2 ·4H2O .............................................30.0mg
Na2SeO3 ..................................................20.0mg
NiCl2 ·6H2O..............................................20.0mg
CuCl2 ·2H2O..............................................10.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deinonized water and bring volume to 1.0L. Mix thoroughly.
NaHCO3 Solution:
Composition per 10.0mL:
NaHCO3 .....................................................1.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 10.0mL. Filter sterilize.
Thiamine·HCl Solution:
Composition per 10.0mL:
Thiamine·HCl ............................................20.0μg
Preparation of Thiamine·HCl Solution:  Add thiamine·HCl to
distilled/deionized water and bring volume to 10.0mL. Filter sterilize.
Preparation of Medium: Add components, except NaHCO3  solu-
tion and thiamine·HCl solution, to distilled/deionized water and bring
volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 10.0mL of sterile NaHCO3  solution and 10.0mL of
sterile thiamine·HCl solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation of Carbophilus carboxidus and Zavarzinia
compransoris.