Carbon Utilization Test

[所属分类:培养基配方] [发布时间:2020-4-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Carbon Utilization Test

培养基配方

Composition per liter:
Ionagar................................................................10.0g
NH4Cl ..................................................................1.0g
MgSO4 ·7H2O............................................................0.5g
Ferric ammonium citrate................................................0.05g
CaCl2 .................................................................0.5mg
Sodium potassium phosphate
buffer (0.33M solution, pH 6.8)........................................1.0L
Carbon source........................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Carbon Source:
Composition per 10.0mL:
Carbon source..........................................................1.0g
Preparation of Carbon Source: Add carbon source to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except carbon source,
to distilled/deionized water and bring volume to 990.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile carbon
source. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.

Use: For the cultivation and differentiation of Pseudomonas species
based on their ability to utilize a specific carbon source.