Carboxymethyl Cellulose Medium (DSMZ Medium 1111)

[所属分类:培养基配方] [发布时间:2020-4-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Carboxymethyl Cellulose Medium
(DSMZ Medium 1111)

培养基配方
Composition per liter:
Carboxymethyl cellulose....................................15.0g
Agar........................................................6.0g
Casitone ...................................................2.0g
(NH4)2SO4 ..................................................1.0g
KH2PO4 .....................................................1.0g
MgSO4 ·7H2O................................................1.0g
CaCl2 ·2H2O ...............................................1.0g
FeCl3 ·6H2O................................................0.2g
KH2PO4  solution..........................................10.0mL
pH 7.0 ± 0.2 at 25°C
KH2PO4 Solution :
Composition per 10.0mL:
KH2PO4 .....................................................1.0g
Preparation of KH2PO4 Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except KH2PO4 solu-
tion, to distilled/deionized water and bring volume to 990.0L. Mix
thoroughly. Gently heat while stirring and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Aeptically add 10.0mL KH2PO4  solution. Mix thoroughly. Pour into
Petri dishes or leave in tubes.
Use:  For the cultivation of Cellvibrio japonicus.