Cardiobacterium hominis Medium

[所属分类:培养基配方] [发布时间:2020-4-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cardiobacterium hominis Medium

培养基配方
Composition per liter:
Glucose .................................................5.0g
Leucine.................................................0.43g
Threonine...............................................0.28g
Glutamic acid............................................0.2g
Valine..................................................0.19g
Glycine.................................................0.18g
Arginine ...............................................0.16g
Histidine...............................................0.13g
Proline..................................................0.1g
Tyrosine................................................0.04g
Buffered salts solution...............................100.0mL
Vitamin solution.......................................10.0mL
pH 7.0 ± 0.2 at 25°C
Buffered Salts Solution:
Composition per liter:
Na2PHO4 ................................................284.0.g
KH2PO4 .................................................272.0.g
NaCl......................................................5.0g
FeSO4 ·7H2O..............................................4.0g
MgSO4 ·7H2O..............................................4.0g
ZnSO4 ·7H2O..............................................0.4g
MnSO4 ·H2O...............................................0.3g
CuSO4 ·5H2O.............................................0.05g
Preparation of Buffered Salts Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl.........................................2.0mg
Calcium pantothenate ...................................1.0mg
Nicotinamide............................................1.0mg
Thiamine·HCl ..........................................1.0mg
Biotin .................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Fil-
ter sterilize.
Use: For the isolation and cultivation of Cardiobacterium hominis.