Carnitine Medium for Torulopsis

[所属分类:培养基配方] [发布时间:2020-4-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Carnitine Medium for Torulopsis

培养基配方
Composition per liter:
Glucose .....................................................20.0g
Agar ........................................................15.0g
L -Asparagine·H2O............................................1.0g
KH2PO4 .......................................................0.5g
MgSO4 ·7H2O..................................................0.5g
NaCl..........................................................0.1g
L -Phenylalanine............................................80.0mg
DL -Tryptophan .............................................50.0mg
DL -Methionine .............................................20.0mg
Adenine.....................................................10.0mg
Cytosine....................................................10.0mg
Inositol....................................................10.0mg
Calcium  D -(+)-pantothenate.................................2.0mg
Thiamine·HCl ...............................................2.0mg
Pyridoxine·HCl..............................................2.0mg
Nicotinic acid...............................................2.0mg
DL -Carnitine·HCl ..........................................1.0mg
Choline......................................................1.0mg
Biotin ....................................................20.0μg
pH 5.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Gently heat and bring to boiling. Ad-
just pH to 5.0. Distribute into tubes or flasks. Autoclave for 15 min at
15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Candida pintolopesii.