Carrot Potato Dextrose Agar (ATCC Medium 1829)

[所属分类:培养基配方] [发布时间:2020-4-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Carrot Potato Dextrose Agar
(ATCC Medium 1829)

培养基配方
Composition per liter:
Agar......................................................25.0g
Glucose...................................................20.0g
Pancreatic digest of casein................................2.5g
Yeast extract..............................................0.5g
MgSO4 ·7H2O...............................................0.3g
CaCO3 .....................................................0.2g
Potatoes, infusion from.................................500.0mL
Carrot juice (any commercial brand).....................15.0 mL
pH 5.6 ± 0.2 at 25°C
Source:  Potato dextrose agar, without carrot juice, is available as a
premixed powder from BD Diagnostic Systems.
Potato Infusion:
Composition per 500.0mL:
Potatoes........................................................300.0g
Preparation of Potato Infusion:  Peel and dice potatoes. Add
500.0mL of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 30 min. Filter through cheesecloth. Reserve fil-
trate.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of yeasts and molds and to induce sporula-
tioni.