​Casamino Acids Glucose Medium

[所属分类:培养基配方] [发布时间:2020-4-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Casamino Acids Glucose Medium
(CAGV Medium)

培养基配方
Composition per liter:
Agar........................................................20.0g
Glucose......................................................1.0g
Vitamin-free casamino acids..................................1.0g
Solution A (mineral salts).................................20.0mL
Vitamin solution...........................................10.0mL
pH 7.2 ± 0.2 at 25°C
Solution A (Mineral Salts):
Composition per liter:
MgSO4 ·7H2O................................................29.7g
NaMoO4 ·2H2O..............................................12.67g
Nitrilotriacetic acid.......................................10.0g
CaCl2 ·2H20 ...............................................3.34g
FeSO4 ·7H2O.................................................0.1g
Solution B (metallic salts) ...............................50.0mL
Preparation of Solution A (Mineral Salts):  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Readjust pH to 7.2 with
H2SO4 or KOH. Add distilled/deionized water to 1.0L.
Solution B (Metallic Salts):
Composition per 100.0mL:
ZnSO4 ·7H2O................................................1.1g
FeSO4 ·7H20 ...............................................0.5g
Ethylenediaminetetraacetic acid.............................0.3g
MnSO4 ·H2O.................................................0.3g
CuSO4 ·5H2O...............................................0.04g
CoCL2 ·6H20...............................................0.02g
Na2B4O7 ·10H20 ...........................................0.02g
Preparation of Solution B (Metallic Salts):  Add a few drops of
H2SO4 to distilled/deionized water to inhibit precipitate formation.
Add components to acidified distilled/deionized water and bring vol-
ume to 100.0mL. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCL............................................0.01g
Calcium pantothenate ......................................5.0mg
Nicotinamide...............................................5.0mg
Riboflavin ................................................5.0mg
Thiamine·HCl .............................................5.0mg
Biotin ....................................................2.0mg
Folic acid.................................................2.0mg
Vitamin B 12 ..............................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Microcyclus aquaticus.