Casamino Acids Medium

[所属分类:培养基配方] [发布时间:2020-4-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Casamino Acids Medium

培养基配方
Composition per liter:
Casamino acids.............................................1.0g
Glucose ...................................................1.0g
Biotin ..................................................0.02mg
Modified Hutner’s basal salts ..........................20.0mL
Modified Hutner’s Basal Salts:
Composition per liter:
MgSO4 ·7H2O..............................................29.7g
Nitrilotriacetic acid.....................................10.0g
CaCl2 ·2H2O..............................................3.34g
FeSO4 ·7H2O...............................................0.1g
(NH4)2MoO4 ..............................................9.25mg
Metals “44”............................................50.0mL
Preparation of Modified Hutner’s Basal Salts: Add nitrilotria-
cetic acid to 500.0mL of distilled/deionized water. Dissolve by adjust-
ing pH to 6.5 with KOH. Add remaining components. Add distilled/
deionized water to 1.0L.
Metals “44”:
Composition per 100.0mL:
ZnSO4 ·7H2O...............................................1.1g
FeSO4 ·7H2O...............................................0.5g
EDTA......................................................0.25g
MnSO4 ·7H2O.............................................0.154g
CuSO4 ·5H2O..............................................0.04g
Co(NO3)2 ·6H2O .........................................0.025g
Na2B4O7 ·10H2O..........................................0.018g
Preparation of Metals “44”: Acidify distilled/deionized water
with a drop of H2SO4 to retard precipitation of salts. Add components
to distilled/deionized water and bring volume to 100.0mL.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For cultivation of Ancylobacter aquaticus and Enhydrobacter
aerosaccus.