Casamino Acids Yeast Extract Broth (CYE Broth)

[所属分类:培养基配方] [发布时间:2020-4-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Casamino Acids Yeast Extract Broth
(CYE Broth)

培养基配方
Composition per liter:
Casamino acids.............................................30.0g
Yeast extract...............................................4.0g
K2HPO4 .....................................................0.5g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Vibrio species from foods.
Casamino Acids Yeast Extract Lincomycin Medium
Composition per liter:
Casamino acids..............................................20.0g
K2HPO4 .....................................................8.71g
Yeast extract................................................6.0g
NaCl.........................................................2.5g
Lincomycin solution.........................................5.0mL
Trace salts solution .......................................1.0mL
pH 8.5 ± 0.2 at 25°C
Lincomycin Solution:
Composition per 5.0mL:
Lincomycin................................................45.0mg
Preparation of Lincomycin Solution: Add lincomycin to dis-
tilled/deionized water and bring volume to 5.0mL. Mix thoroughly. Fil-
ter sterilize.
Trace Salts Solution:
Composition per liter:
MgSO4 ·7H2O.................................................50.0g
MnCl2 · 4H2O.................................................5.0g
FeCl2 ........................................................5.0g
Preparation of Trace Salts Solution:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Add sufficient 0.1N
H2SO4 to dissolve components. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except trace salts solu-
tion and lincomycin solution, to distilled/deionized water and bring
volume to 994.0mL. Mix thoroughly. Adjust pH to 8.5. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 1.0mL
of sterile trace salts solution and 5.0mL of sterile lincomycin solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of heat-labile, toxin-producing enterotoxi-
genic Escherichia coli.