Casman Agar Base

[所属分类:培养基配方] [发布时间:2020-4-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Casman Agar Base

培养基配方
Composition per liter:
Noble agar................................................14.0g
Proteose peptone No.3.....................................10.0g
Tryptose .................................................10.0g
NaCl.......................................................5.0g
Beef extract...............................................3.0g
Cornstarch.................................................1.0g
Glucose ...................................................0.5g
p-Aminobenzoic acid.......................................0.05g
Nicotinamide..............................................0.05g
Blood....................................................50.0mL
Water-lysed blood solution ...............................1.5mL
pH 7.3 ± 0.2 at 25°C
Water-Lysed Blood Solution:
Composition per 8.0mL:
Blood.....................................................2.0mL
Preparation of Water-Lysed Blood Solution: Add blood to dis-
tilled/deionized water and bring volume to 8.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components, except blood and wa-
ter-lysed blood solution, to distilled/deionized water and bring volume
to 948.5mL. Mix thoroughly. Gently heat to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 50.0mL
of sterile blood and 1.5mL of sterile water-lysed blood solution (one
part blood to three parts water). Water-lysed blood may be omitted if
sterile blood is partially lysed due to storage. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation of fastidious bacteria from clinical specimens.
For the cultivation under reduced oxygen tension of fastidious micro-
organisms such as Haemophilus influenzae, Neisseria meningitidis,
and Neisseria gonorrhoeae.