Castenholz D Medium, Modified (Medium D, Modified)

[所属分类:培养基配方] [发布时间:2020-4-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Castenholz D Medium, Modified
(Medium D, Modified)

培养基配方
Composition per liter:
NaCl...........................................................160.0g
NaNO3 ..........................................................0.69g
Na2HPO4 .......................................................0.111g
KNO3 ..........................................................0.103g
MgSO4 ·7H2O.....................................................0.1g
Nitrilotriacetic acid............................................0.1g
CaSO4 ·2H2O....................................................0.06g
FeCl3 ..........................................................0.3mg
Trace metal solution, Castenholz................................1.0mL
pH 7.5 ± 0.2 at 25°C
Trace Metal Solution, Castenholz:
Composition per liter:
MnSO4 ·H2O.....................................................2.28g
H3BO3 ...........................................................0.5g
ZnSO4 ·7H2O.....................................................0.5g
Co(NO3)2 ·6H2O ...............................................0.025g
CuSO4 ·5H2O...................................................0.025g
Na2MoO4 ·2H2O ................................................0.025g
H2SO4 ..........................................................0.5mL
Preparation of Trace Metal Solution, Castenholz: Add com-
ponents to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly.
Preparation of Medium: Add nitrilotriacetic acid to 500.0mL of dis-
tilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add re-
maining components. Mix thoroughly. Readjust pH to 7.5. Bring volume
to 1.0L with distilled/deionized water. Mix thoroughly. Distribute into
screw-capped tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the isolation of halophilic cyanobacteria.