Castenholz ND Medium (Medium ND)

[所属分类:培养基配方] [发布时间:2020-4-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Castenholz ND Medium
(Medium ND)

培养基配方
Composition per liter:
Na2HPO4 ....................................................0.11g
MgSO4 ·7H2O.................................................0.1g
Nitrilotriacetic acid........................................0.1g
CaSO4 ·2H2O................................................0.06g
NaCl........................................................8.0mg
FeCl3 solution..............................................1.0mL
Micronutrient solution......................................0.5mL
pH 7.5 ± 0.2 at 25°C
FeCl 3 Solution:
Composition per liter:
FeCl3 ·6H2O.................................................2.28g
Preparation of FeCl3 Solution: Add FeCl3 ·6H2O to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Micronutrient Solution:
Composition per liter:
MnSO4 ·H2O.................................................2.28g
H3BO3 .......................................................0.5g
ZnSO4 ·7H2O.................................................0.5g
CoCl2 ·6H2O...............................................0.025g
CuSO4 ·5H2O...............................................0.025g
Na2MoO4 ·2H2O.............................................0.025g
H2SO4 ......................................................0.5mL
Preparation of Micronutrient Solution:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add nitrilotriacetic acid to 500.0mL of
distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH.
Add remaining components. Mix thoroughly. Readjust pH to 8.2.
Bring volume to 1.0L with distilled/deionized water. Mix thoroughly.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the isolation of cyanobacteria, including thermophilic spe-
cies, that require reduced nitrogen concentrations.