Shandong Tuopu Biol-Engineering Co.,Ltd
Castenholz TYE Medium
(Castenholz Trypticase ™
Yeast Extract Medium)
培养基配方
Composition per liter:
Castenholz salts, 2X......................................500.0mL
1% TYE....................................................100.0mL
pH 7.6 ± 0.2 at 25°C
Castenholz Salts, 2X:
Composition per liter:
Agar ......................................................30.0g
NaNO3 ......................................................1.4g
Na2HPO4 ...................................................0.22g
KNO3 ......................................................0.21g
Nitrilotriacetic acid.......................................0.2g
MgSO4 ·7H2O................................................0.2g
CaSO4 ·2H2O...............................................0.12g
NaCl......................................................0.016g
FeCl3 (0.03% solution) ....................................2.0mL
Nitsch’s trace elements ..................................2.0mL
Preparation of Castenholz Salts, 2X: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Adjust pH to 8.2. Autoclave for 15 min at
15 psi pressure–121°C.
Nitsch’s Trace Elements:
Composition per liter:
MnSO4 .......................................................2.2g
H3BO3 .......................................................0.5g
ZnSO4 .......................................................0.5g
CoCl2 ·6H2O ..............................................0.046g
Na2MoO4 ...................................................0.025g
CuSO4 .....................................................0.016g
H2SO4 ......................................................0.5mL
Preparation of Nitsch’s Trace Elements: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
1% TYE
Composition per liter:
Pancreatic digest of casein.................................10.0g
Yeast extract...............................................10.0g
Preparation of 1% TYE: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 500.0mL of sterile
Castenholz salts, 2X, 100.0mL of sterile 1% TYE, and 400.0mL of
sterile distilled/deionized water. Adjust pH to 7.6.
Use: For the cultivation and maintenance of Thermonema lapsum and
Thermus species.
