Castenholz TYE Medium with 2% Trypticase ™ Yeast Extract

[所属分类:培养基配方] [发布时间:2020-4-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Castenholz TYE Medium
with 2% Trypticase ™ Yeast Extract

培养基配方
Composition per liter:
Castenholz salts, 2X........................................500.0mL
2% TYE .....................................................100.0mL
pH 7.6 ± 0.2 at 25°C
Castenholz Salts, 2X:
Composition per liter:
Agar .........................................................30.0g
NaNO3 .........................................................1.4g
Na2HPO4 ......................................................0.22g
KNO3 .........................................................0.21g
MgSO4 ·7H2O...................................................0.2g
Nitrilotriacetic acid..........................................0.2g
CaSO4 ·2H2O..................................................0.12g
NaCl.........................................................0.016g
FeCl3 solution (0.03% solution) ..............................2.0mL
Nitsch’s trace elements .....................................2.0mL
Preparation of Castenholz Salts, 2X: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Adjust pH to 8.2. Autoclave for 15 min at
15 psi pressure–121°C.
Nitsch’s Trace Elements:
Composition per liter:
MnSO4 .........................................................2.2g
H3BO3 .........................................................0.5g
ZnSO4 .........................................................0.5g
CoCl2 ·6H2O.................................................0.046g
Na2MoO4 .....................................................0.025g
CuSO4 .......................................................0.016g
H2SO4 ........................................................0.5mL
Preparation of Nitsch’s Trace Elements: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
2% TYE
Composition per liter:
Pancreatic digest of casein....................................20.0g
Yeast extract..................................................20.0g
Preparation of 2% TYE: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 500.0mL of sterile
Castenholz salts, 2X, 100.0mL of sterile 2% TYE, and 400.0mL of
sterile distilled/deionized water. Adjust pH to 7.6.
Use:  For the cultivation and maintenance of Thermus species.