CC Medium

[所属分类:培养基配方] [发布时间:2020-4-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CC Medium

培养基配方
Composition per liter:
Agar.........................................................20.0g
KH2PO4 .......................................................4.0g
Potato starch.................................................0.5g
Solution 3.................................................100.0mL
Solution 1..................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Solution 1:
Composition per liter:
MgSO4 ·7H2O..................................................20.0g
CaCl2 ·2H2O ..................................................2.0g
FeSO4 ·7H2O...................................................0.4g
H3BO3 ........................................................0.02g
MnSO4 ·2H2O.................................................0.015g
NaMoO4 ·2H2O................................................0.015g
KI............................................................0.01g
ZnSO4 ........................................................4.0mg
CoCl2 ·4H2O .................................................0.4mg
CuSO4 ·5H2O..................................................0.4mg
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH with
10.0mL of 10% HCl solution.
Solution 3:
Composition per 100.0mL:
Pancreatic digest of casein...................................12.0g
Yeast extract.................................................12.0g
L- Cysteine·HCl...............................................0.5g
L- Asparagine ................................................0.03g
DL- Tryptophan................................................0.02g
Solution 2...................................................12.0mL
Preparation of Solution 3: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Solution 2:
Composition per 100.0mL:
p-Aminobenzoic acid.........................................0.02g
Calcium pantothenate .......................................0.02g
m-Inositol..................................................0.02g
Pyridoxine·HCl.............................................0.02g
Thiamine·HCl ..............................................0.02g
Nicotinamide................................................0.01g
Nicotinic acid..............................................0.01g
Folic acid..................................................5.0mg
Biotin .....................................................1.0mg
Vitamin B 12 ...............................................1.0mL
Preparation of Solution 2: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add KH2PO4 to distilled/deionized water
and bring volume to 250.0mL. Mix thoroughly. Adjust pH to 7.6 with
NaOH. Add 10.0mL of solution 1. In a separate flask, add potato starch
to 70.0mL of boiling distilled/deionized water. Add potato starch solu-
tion to other solution. Add agar. Bring volume to 900.0mL of distilled/
deionized water. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 100.0mL of sterile solution 3. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Actinomycetes species.