CCD Agar with Pyruvate and Cefazolin (Blood-free Selective Medium)

[所属分类:培养基配方] [发布时间:2020-4-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CCD Agar with Pyruvate and Cefazolin
(Blood-free Selective  Medium)

培养基配方
Composition per liter:
Agar ........................................................12.0g
Beef extract.................................................10.0g
Peptone......................................................10.0g
NaCl..........................................................5.0g
Charcoal, bacteriological.....................................4.0g
Casein hydrolysate............................................3.0g
Sodium deoxycholate solution................................10.0mL
FeSO4 solution...............................................5.0mL
Sodium pyruvate solution.....................................5.0mL
Cefazolin solution...........................................1.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium, without deoxycholate and cefazolin solutions,
is available as a premixed powder from HiMedia.
FeSO4 Solution:
Composition per 10.0mL:
FeSO4 .......................................................0.5g
Preparation of  FeSO4 Solution: Add FeSO4 to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool 25°C.
Sodium Pyruvate Solution:
Composition per 10.0mL:
Sodium pyruvate .............................................0.5g
Preparation of Sodium Pyruvate Solution:  Add sodium pyru-
vate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Gently heat while stirring and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool 25°C.
Sodium Deoxycholate Solution:
Composition per 100.0mL:
Sodium deoxycholate......................................10.0g
Preparation of Sodium Deoxycholate Solution: Add sodium
deoxycholate to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Gently heat while stirring and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool 25°C.
Cefazolin Solution:
Composition per 10.0mL:
Cefazolin...................................................0.1g
Preparation of Cefazolin Solution: Add cefazolin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium:  Add components, except cefazolin solu-
tion and sodium deoxycholate solution, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Heat with frequent ag-
itation and boil for 1 min to completely dissolve. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 50°–55°C. Add 10.0mL of sterile so-
dium deoxycholate solution and 1.0mL of sterile cefazolin solution.
Mix thoroughly. Pour into sterile Petri dishes.
Use:  For the selective isolation of Campylobacter species, especially
Campylobacter jejuni from human feces.