CCY Modified Medium

[所属分类:培养基配方] [发布时间:2020-4-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CCY Modified Medium

培养基配方
Composition per liter:
Yeast extract...............................................30.0g
Casamino acids..............................................20.0g
Na2HPO4 ....................................................2.48g
KH2PO4 .....................................................0.41g
MgSO4 ·7H2O...............................................20.0mg
MnSO4 ·H2O.................................................7.5mg
Citric acid.................................................6.4mg
FeSO4 ·7H2O................................................6.4mg
Sodium pyruvate solution.................................100.00mL
pH 7.3 ± 0.2 at 25°C
Sodium Pyruvate Solution:
Composition per 100.0mL:
Sodium pyruvate..............................................23.2g
Preparation of Sodium Pyruvate Solution:  Add sodium pyru-
vate to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except sodium pyru-
vate solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Adjust pH to 7.3. Autoclave for 15 min at
15 psi pressure–121°C. Aseptically add 100.0mL of sterile sodium
pyruvate solution. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the cultivation of Staphylococcus aureus.