CDC Anaerobe Blood Agar

[所属分类:培养基配方] [发布时间:2020-4-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CDC Anaerobe Blood Agar

培养基配方
Composition per liter:
Agar.........................................................20.0g
Pancreatic digest of casein..................................15.0g
Papaic digest of soybean meal.................................5.0g
NaCl..........................................................5.0g
Yeast extract.................................................5.0g
L- Cystine....................................................0.4g
Sheep blood, defibrinated...................................50.0mL
Vitamin K 1 solution.........................................1.0mL
Hemin solution...............................................0.5mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................1.0g
Ethanol.......................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin ..........................................................1.0g
NaOH (1N solution)............................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Medium:  Add components, except vitamin K 1 and
sheep blood, to distilled/deionized water and bring volume to
949.0mL. Mix thoroughly. Heat gently and bring to boiling for 1 min.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 1.0mL of vitamin K 1 solution and 50.0mL of sterile,
defibrinated sheep blood. Mix thoroughly. Pour into sterile Petri dish-
es.
Use: For the isolation and cultivation of fastidious and slow-growing,
obligate anaerobic bacteria from a variety of clinical and nonclinical
specimens. For the isolation and cultivation of Actinomyces israelii,
Bacteroides melaninogenicus, Bacteroides thetaiotaomicron, Clostrid-
ium haemolyticum, and Fusobacterium necrophorum.