CDC Anaerobe Blood Agar with Kanamycin and Vancomycin

[所属分类:培养基配方] [发布时间:2020-4-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CDC Anaerobe Blood Agar
with Kanamycin and Vancomycin

培养基配方
Composition per liter:
Agar...........................................................20.0g
Pancreatic digest of casein....................................15.0g
NaCl............................................................5.0g
Papaic digest of soybean meal...................................5.0g
Yeast extract...................................................5.0g
L- Cystine......................................................0.4g
Sheep blood, defibrinated.....................................50.0mL
Antibiotic solution...........................................10.0mL
Vitamin K 1 solution...........................................1.0mL
Hemin solution.................................................0.5mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
Antibiotic Solution:
Composition per 10.0mL:
Kanamycin......................................................0.1g
Vancomycin ...................................................7.5mg
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ...................................................1.0g
Ethanol......................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin..........................................................1.0g
NaOH (1N solution)...........................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Medium:  Add components, except vitamin K 1 so-
lution and sheep blood, to distilled/deionized water and bring volume
to 949.0mL. Mix thoroughly. Heat gently and bring to boiling for 1
min. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–
55°C. Aseptically add 1.0mL of sterile vitamin K 1 solution and
50.0mL of sterile, defibrinated sheep blood. Mix thoroughly. Pour into
sterile Petri dishes.
Use: For the selective isolation of fastidious and slow-growing, obli-
gate anaerobic Gram-negative bacteria, especially Bacteroides species,
from a variety of clinical and nonclinical specimens.