CDC Anaerobe Blood Agar with Phenylethyl Alcohol (CDC Anaerobe Blood Agar with PEA)

[所属分类:培养基配方] [发布时间:2020-4-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CDC Anaerobe Blood Agar
with Phenylethyl Alcohol
(CDC Anaerobe Blood Agar with PEA)

培养基配方
Composition per liter:
Agar .......................................................20.0g
Pancreatic digest of casein.................................15.0g
NaCl.........................................................5.0g
Papaic digest of soybean meal................................5.0g
Yeast extract................................................5.0g
L- Cystine ..................................................0.4g
Sheep blood, defibrinated..................................50.0mL
Vitamin K 1 solution ......................................10.0mL
Hemin solution..............................................0.5mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ...............................................0.1g
Phenylethyl alcohol.......................................25.0g
Ethanol..................................................74.0mL
Preparation of Vitamin K 1 Solution:  Add components to
74.0mL of absolute ethanol. Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin .....................................................1.0g
NaOH (1N solution).......................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Medium:  Add components, except vitamin K 1 so-
lution and sheep blood, to distilled/deionized water and bring volume
to 940.0mL. Mix thoroughly. Heat gently and bring to boiling for 1
min. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–
55°C. Aseptically add 1.0mL of vitamin K 1 solution and 50.0mL of
sterile, defibrinated sheep blood. Mix thoroughly. Pour into sterile Petri
dishes.
Use: For the selective isolation of fastidious and slow-growing, obli-
gate anaerobic bacteria from a variety of clinical and nonclinical spec-
imens.