CDC Anaerobe Laked Blood Agar with Kanamycin and Vancomycin (CDC Anaerobe Laked Blood Agar with KV)

[所属分类:培养基配方] [发布时间:2020-4-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CDC Anaerobe Laked Blood Agar
with Kanamycin and Vancomycin
(CDC Anaerobe Laked Blood Agar with KV)

培养基配方
Composition per liter:
Agar........................................................20.0g
Pancreatic digest of casein.................................15.0g
Papaic digest of soybean meal................................5.0g
Yeast extract................................................5.0g
L- Cystine...................................................0.4g
Sheep blood, defibrinated, laked ..........................50.0mL
Antibiotic solution........................................10.0mL
Vitamin K 1 solution........................................1.0mL
Hemin solution..............................................0.5mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
Antibiotic Solution:
Composition per 10.0mL:
Kanamycin...................................................0.1g
Vancomycin..................................................7.5mg
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ...................................................1.0g
Ethanol......................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin .........................................................1.0g
NaOH (1N solution)...........................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Medium:  Add components, except antibiotic solu-
tion, vitamin K 1 , and laked sheep blood, to distilled/deionized water
and bring volume to 939.0mL. Mix thoroughly. Heat gently and bring
to boiling for 1 min. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°–55°C. Aseptically add 1.0mL of sterile vitamin K 1 solu-
tion and 10.0mL of sterile antibiotic solution. Mix thoroughly. Asepti-
cally add 50.0mL of sterile, defibrinated, laked sheep blood. Laked
blood is prepared by freezing whole blood overnight and thawing to
room temperature. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the selective isolation of fastidious and slow-growing, obli-
gate anaerobic bacteria from a variety of clinical and nonclinical spec-
imens.