Cellobiose Polymyxin B Colistin Agar, Modified

[所属分类:培养基配方] [发布时间:2020-4-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellobiose Polymyxin
B Colistin Agar, Modified

培养基配方
Composition per liter:
Solution 1................................................900.0mL
Solution 2................................................100.0mL
pH 7.6 ± 0.2 at 25°C
Solution 1:
Composition per 900.0mL:
NaCl.........................................................20.0g
Agar.........................................................15.0g
Peptone .....................................................10.0g
Beef extract..................................................5.0g
1000× dye stock solution....................................1.0mL
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 7.6.
Gently heat and bring to boiling. Do not autoclave. Cool to 48°–55°C.
1000X Dye Stock Solution:
Composition per 100.0mL:
Bromthymol Blue..............................................4.0g
Cresol Red ..................................................4.0g
Ethanol (95% solution)....................................100.0mL
Preparation of 1000X Dye Stock Solution: Add Bromthymol
Blue and Cresol Red to 100.0mL of ethanol. Mix thoroughly.
Solution 2:
Composition per 100.0mL:
Cellobiose..................................................10.0g
Colistin..................................................400,000U
Polymyxin B...............................................100,000U
Preparation of Solution 2: Add cellobiose to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Gently heat until
dissolved. Cool to 25°C. Add colistin and polymyxin B. Mix thoroughly.
Preparation of Medium: Combine cooled solution 1 and solution
2. Mix thoroughly. Do not autoclave. Pour into sterile Petri dishes.
Use:  For the cultivation of Vibrio species from foods.