Cellulolytic Agar with Sea Salts

[所属分类:培养基配方] [发布时间:2020-4-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellulolytic Agar with Sea Salts

培养基配方
Composition per liter:
Agar ...........................................................20.0g
NH4Cl ...........................................................2.0g
K2HPO4 .........................................................1.65g
Yeast extract....................................................1.2g
L -Cysteine·HCl·H2O ...........................................0.5g
Cellulose suspension..........................................200.0mL
Filtered seawater.............................................200.0mL
Mineral solution..............................................150.0mL
Resazurin (0.1% solution).......................................1.0mL
pH 7.2 ± 0.2 at 25°C
Cellulose Suspension:
Composition per 200.0mL:
Cellulose powder, Whatman CF11...................................8.0g
Preparation of Cellulose Suspension: Add cellulose powder to
200.0mL of distilled/deionized water and mix thoroughly.
Mineral Solution:
Composition per liter:
NaCl...............................................................6.0g

(NH4)2SO4 .........................................................6.0g
CaCl2 .............................................................0.6g
MgSO4 .............................................................0.6g
Preparation of Mineral Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Prepare and dispense medium anaerobi-
cally under 100% N2 . Add components to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2 with 5M
NaOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C.
Use:  For the cultivation and maintenance of Clostridium papyrosol-
vens and other marine bacteria that can utilize cellulose as a carbon
source.