Cellulolytic Broth with Sea Salts

[所属分类:培养基配方] [发布时间:2020-4-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellulolytic Broth with Sea Salts

培养基配方
Composition per liter:
K2HPO4 .....................................................1.65g
NH4Cl .......................................................1.0g
Yeast extract................................................0.6g
L -Cysteine·HCl·H2O .......................................0.5g
Filtered seawater.........................................200.0mL
Mineral solution..........................................150.0mL
Resazurin (0.1% solution)...................................1.0mL
pH 7.2 ± 0.2 at 25°C
Mineral Solution:
Composition per liter:
NaCl.........................................................6.0g
(NH4)2SO4 ...................................................6.0g
CaCl2 .......................................................0.6g
MgSO4 .......................................................0.6g
Preparation of Mineral Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Prepare and dispense medium anaerobi-
cally in 100% N2 atmosphere. Add components to distilled/deionized
water and bring volume to 1.0L. Adjust pH to 7.2 with 5M NaOH. Dis-
tribute into tubes or flasks that contain cellulose as a strip (4.5cm ×
1.0cm) of Whatman No. 1 filter paper. Autoclave for 15 min at 15 psi
pressure–121°C.
Use:  For the cultivation and maintenance of Clostridium papyrosol-
vens and other marine bacteria that can utilize cellulose as a carbon
source.