Cellulolytic Medium

[所属分类:培养基配方] [发布时间:2020-4-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellulolytic Medium

培养基配方
Composition per liter:
NaHCO3 ......................................................2.06g
Cellulose ....................................................2.0g
NH4Cl .......................................................0.68g
K2HPO4 .....................................................0.296g
KH2PO4 ......................................................0.18g
(NH4)2SO4 ...................................................0.15g
MgSO4 ·7H2O.................................................0.12g
CaCl2 ·2H2O................................................61.0mg
FeSO4 ·7H2O................................................21.0mg
Nitrilotriacetic acid.......................................15.0mg
NaCl........................................................10.0mg
MnSO4·H2O...................................................5.0mg
CoCl2 ·H2O .................................................1.0mg
Resazurin ...................................................1.0mg
ZnSO4 ·7H2O.................................................1.0mg
CuSO4 ·5H2O.................................................0.1mg
H3BO3 .......................................................0.1mg
KAl(SO4)2 ·12H2O............................................0.1mg
Na2MoO4 ·2H2O...............................................0.1mg
Wolfe’s vitamin solution...................................10.0mL
L -Cysteine·HCl·H2O solution...............................5.0mL
pH 7.5 ± 0.2 at 25°C
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl............................................10.0mg
Thiamine·HCl ..............................................5.0mg
Riboflavin..................................................5.0mg
Nicotinic acid..............................................5.0mg
Calcium  DL -pantothenate...................................5.0mg
p-Aminobenzoic acid.........................................5.0mg
Thioctic acid ..............................................5.0mg
Biotin......................................................2.0mg
Folic acid .................................................2.0mg
Cyanocobalamin...........................................100.0μg
Preparation of Wolfe’s Vitamin Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
L -Cysteine·HCl·H2O Solution:
Composition per 20.0mL:
L -Cysteine·HCl·H2O.........................................1.0g
Preparation of  L -Cysteine·HCl·H2O Solution: Add  L -
cysteine·HCl·H2O to distilled/deionized water and bring volume to
20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except Wolfe’s vitamin
solution and  L -cysteine·HCl·H2O solution, to distilled/deionized water and
bring volume to 985.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 10.0mL of sterile Wolfe’s vitamin solu-
tion and 5.0mL of sterile  L -cysteine·HCl·H2O solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of cellulolytic bacteria.