Cellulolytic Medium with Rumen Fluid and Soluble Starch

[所属分类:培养基配方] [发布时间:2020-4-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellulolytic Medium with
Rumen Fluid and Soluble Starch

培养基配方
Composition per liter:
Basal medium ...........................................975.0mL
Alkaline solution........................................25.0mL
pH 6.8 ± 0.2 at 25°C
Basal Medium:
Composition per 975.0mL:
Agar .....................................................15.0g
NaHCO3 ...................................................6.37g
Pancreatic digest of casein................................5.0g
Cellobiose ................................................5.0g
Soluble starch.............................................5.0g
NaCl.......................................................0.9g
(NH4)2SO4 .................................................0.9g
K2HPO4 ...................................................0.45g
KH2PO4 ...................................................0.45g
MgSO4 ·7H2O..............................................0.18g
CaCl2 ....................................................0.09g
Resazurin ................................................1.0mg
Preparation of Basal Medium: Add components to distilled/de-
ionized water and bring volume to 975.0mL. Mix thoroughly. Gently
heat and bring to boiling under a gas phase of 98% CO2 + 2% H2 . Cool
slightly.
Alkaline Solution:
Composition per 25.0mL:
L -Cysteine·HCl·H2O .......................................0.25g
Na2S·9H2O...................................................0.25g
Preparation of Alkaline Solution: Add components to 25.0mL of
distilled/deionized water. Mix thoroughly. Prepare freshly.
Preparation of Medium: Prepare 975.0mL of basal medium. Heat
to boiling and cool to 25°C. Add 25.0mL of freshly prepared alkaline
solution. Distribute into tubes using anaerobic techniques under a gas
phase of 98% CO2 + 2% H2 . Autoclave for 15 min at 15 psi pressure–
121°C. Adjust pH to 6.8.
Use:  For the cultivation of Selenomonas ruminantium and Succinimo-
nas amylolytica.