Cellulomonas fermentans Medium

[所属分类:培养基配方] [发布时间:2020-4-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellulomonas fermentans Medium

培养基配方
Composition per liter:
Yeast extract.................................................5.0g
K2HPO4 ......................................................2.21g
KH2PO4 .......................................................1.5g
(NH4)2SO4 ....................................................1.3g
MgCl2 ·6H2O .................................................0.1g
CaCl2 ·2H2O ................................................0.02g
FeSO4 ·7H2O................................................1.25mg
Resazurin....................................................1.0mg
Cellobiose solution.........................................50.0mL
NaHCO3  solution............................................10.0mL
L -Cysteine·HCl solution ..................................10.0mL
pH 7.4 ± 0.2 at 25°C
Cellobiose Solution:
Composition per 50.0mL:
D- Cellobiose (or cellulose)..................................5.0g
Preparation of Cellobiose Solution: Add cellobiose (or cellu-
lose) to distilled/deionized water and bring volume to 50.0mL. Mix
thoroughly. Autoclave under 100% N2  for 15 min at 15 psi pressure–
121°C.
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO3 ........................................................0.8g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
under 100% N2  for 15 min at 15 psi pressure–121°C.
L -Cysteine·HCl Solution:
Composition per 10.0mL:
L -Cysteine·HCl ..............................................0.5g
Preparation of  L -Cysteine·HCl Solution: Add  L -cysteine·HCl to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Autoclave under 100% N2  for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except cellobiose solu-
tion, NaHCO3  solution, and  L -cysteine·HCl solution, to distilled/deion-
ized water and bring volume to 930.0mL. Mix thoroughly. Sparge
under 100% N2  for 10 min. Autoclave under 100% N2  for 15 min at 15
psi pressure–121°C. Aseptically and anaerobically add 50.0mL of ster-
ile cellobiose solution, 10.0mL of sterile NaHCO3  solution, and
10.0mL of sterile  L -cysteine·HCl solution. Mix thoroughly.
Use:  For the cultivation and maintenance of Cellulomonas fermen-
tans .