Cellulose Anaerobe Medium (LMG Medium 94)

[所属分类:培养基配方] [发布时间:2020-4-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellulose Anaerobe Medium
(LMG Medium 94)

培养基配方
Composition per liter:
NaHCO3 ......................................................2.1g
Cellulose ...................................................2.0g
NH4Cl ......................................................0.68g
KH2PO4 .....................................................0.18g
(NH4)2SO4 ..................................................0.15g
MgSO4 ·7H2O................................................0.12g
K2HPO4 ...................................................296.0mg
CaCl2 ·2H2O...............................................61.0mg
FeSO4 ·7H2O...............................................21.0mg
Nitrilotriacetic acid......................................15.0mg
NaCl.......................................................10.0mg
MnSO4 ·H2O.................................................5.0mg
CoCl2 ·6H2O ...............................................1.0mg
ZnSO4 ·7H2O................................................1.0mg
Resazurin ..................................................1.0mg
CuSO4 ·5H2O................................................0.1mg
KAl(SO4)2 ·12H2O...........................................0.1mg
H3BO3 ......................................................0.1mg
Na2MoO4 ·2H2O .............................................0.1mg
Vitamin solution............................................5.0mL
Cysteine hydrochloride solution.............................5.0mL
pH 7.1 ± 0.2 at 25°C
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl............................................10.0mg
Calcium  DL -pantothenate...................................5.0mg
Lipoic acid ................................................5.0mg
Lipoic acid ................................................5.0mg
Lipoic acid ................................................5.0mg
Lipoic acid ................................................5.0mg
Lipoic acid ................................................5.0mg
Nicotinic acid..............................................5.0mg
p-Aminobenzoic acid.........................................5.0mg
Riboflavin .................................................5.0mg
Thiamine·HCl ..............................................5.0mg
Biotin .....................................................2.0mg
Folic acid..................................................2.0mg
Vitamin B 12 ...............................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
L -Cysteine Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H2O.......................................0.5g
Preparation of  L -Cysteine Solution:  Add  L -cysteine·HCl·H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Medium: Add nitrilotriacetic acid to 500.0mL of
distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining
components except vitamin solution and cysteine hydrochloride solu-
tion. Add 485.0mL distilled/deionized water. Adjust pH to 7.1. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 10.0mL of sterile vitamin solution and 5.0mL sterile cysteine hy-
drochloride solution. Mix thoroughly. Aseptically distribute to tubes or
flasks.
Use: For the cultivation of cellulose-utilizing anerobic bacteria.