Cellulose Overlay Agar

[所属分类:培养基配方] [发布时间:2020-4-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellulose Overlay Agar

培养基配方
Composition per plate:
Stan 5 agar .............................................15.0mL
Cellulose overlay agar ...................................5.0mL
Stan 5 Agar:
Composition per liter:
Solution B...............................................650.0mL
Solution A...............................................350.0mL
Solution A:
Composition per 350.0mL:
CaCl2 ·2H2O ..............................................1.0g
(NH4)2SO4 .................................................1.0g
MgSO4 ·7H2O...............................................1.0g
Trace elements solution...................................1.0mL
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 350.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Trace Elements Solution:
Composition per liter:
EDTA.......................................................8.0g
MnCl2 ·4H2O ..............................................0.1g
CoCl2 ....................................................0.02g
KBr.......................................................0.02g
ZnCl2 ....................................................0.02g
CuSO4 ....................................................0.01g
H3BO3 ....................................................0.01g
NaMoO4 ·2H2O.............................................0.01g
BaCl2 ....................................................5.0mg
LiCl......................................................5.0mg
SnCl2 ·2H2O..............................................5.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Solution B:
Composition per 650.0mL:
Agar .....................................................10.0g
K2HPO4 ....................................................1.0g
Preparation of Solution B: Add components to distilled/deionized
water and bring volume to 650.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Preparation of Stan 5 Agar: Aseptically combine 350.0mL of
cooled, sterile solution A and 650.0mL of cooled, sterile solution B.
Mix thoroughly.
Cellulose Overlay Agar:
Composition per liter:
Solution A................................................350.0mL
Solution B................................................650.0mL
Solution A:
Composition per 350.0mL:
CaCl2 ·2H2O.................................................1.0g
(NH4)2SO4 ...................................................1.0g
MgSO4 ·7H2O.................................................1.0g
Trace elements solution ....................................1.0mL
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 350.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Trace Elements Solution:
Composition per liter:
EDTA..........................................................8.0g
MnCl2 ·4H2O..................................................0.1g
CoCl2 .......................................................0.02g
KBr..........................................................0.02g
ZnCl2 .......................................................0.02g
CuSO4 .......................................................0.01g
H3BO3 .......................................................0.01g
NaMoO4 ·2H2O................................................0.01g
BaCl2 .......................................................5.0mg
LiCl.........................................................5.0mg
SnCl2 ·2H2O.................................................5.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Solution B:
Composition per 650.0mL:
Agar ........................................................10.0g
K2HPO4 .......................................................1.0g
Preparation of Solution B: Add components to distilled/deionized
water and bring volume to 650.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Preparation of Cellulose Overlay Agar: Aseptically combine
350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile
solution B. Mix thoroughly.
Preparation of Medium: Pour cooled, sterile Stan 5 agar into ster-
ile Petri dishes in 15.0mL volumes. Allow agar to solidify. Overlay
each plate with 5.0mL of cellulose overlay agar.
Use: For the cultivation of myxobacteria.