CENS Medium (DSMZ Medium 748)

[所属分类:培养基配方] [发布时间:2020-4-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CENS Medium
(DSMZ Medium 748)

培养基配方
Composition per liter:
Soytone.......................................................4.0g
NH4Cl ........................................................1.0g
K2HPO4 .......................................................0.9g
KH2PO4 .......................................................0.6g
Na2S2O3 ·5H2O................................................0.5g
MgSO4 ·7H2O..................................................0.2g
CaCl2 ·2H2O ...............................................75.0mg
EDTA.........................................................5.0mg
Vitamin B 12 ...............................................20.0μg
Biotin......................................................15.0μg
Na-pyruvate solution .......................................10.0mL
Chelated iron solution.......................................2.0mL
True Blue trace elements ....................................1.0mL
pH 7.0 ± 0.2 at 25°C
Na-pyruvate Solution:
Composition per 10.0mL:
Na-pyruvate ..................................................2.2g
Preparation of Na-pyruvate Solution: Add Na-pyruvate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Filter sterilize.
True Blue Trace Elements:
Composition per 250mL:
EDTA...........................................................2.5g
MnCl2 ·4H2O ..................................................0.2g
H3BO3 .........................................................0.1g
Na2MoO4 ·4H2O.................................................0.1g
ZnCl2 .......................................................50.0mg
NiCl2 ·6H2O.................................................50.0mg
CoCl2 ·2H2O ................................................20.0mg
CuCl2 ·2H2O ................................................10.0mg
Na2SeO3 ......................................................5.0mg
NaVO3 ·H2O ..................................................5.0mg
Preparation of True Blue Trace Elements: Add components
one at a time to approximately 200.0mL distilled/deionized water.
Bring final volume to 250.0mL with additional distilled/deionized wa-
ter.
Chelated Iron Solution:
Composition per 900mL:
EDTA.........................................................2.0g
FeCl2 ·4H2O.................................................1.0g
HCl.........................................................3.0mL
Preparation of Chelated Iron Solution: Add components to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Preparation of Medium: Add components, except Na-pyruvate so-
lution, to distilled/deionized water and bring volume to 990.0L. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 10.0mL sterile Na-pyruvate solution. Aseptical-
ly distribute into sterile tubes or flasks.
Use:  For the cultivation of Rhodocista centenaria (Rhodospirillum cen-
tenum).